One therapeutic approach for preventing diabetes mellitus and obesity is to retard the absorption of glucose via inhibition of alpha-glucosidase. Two unsaturated fatty acids with strong alpha-glucosidase inhibitory activity, 7(Z)-octadecenoic acid (1) and 7(Z),10(Z)-octadecadienoic acid (2), were purified from the body wall of Stichopus japonicus. IC(50) values of compounds 1 and 2 were 0.51 and 0.67 mug/mL against Saccharomyces cerevisiae alpha-glucosidase and 0.49 and 0.60 mug/mL against Bacillus stearothermophilus alpha-glucosidase, respectively. These compounds mildly inhibited rat-intestinal sucrase and maltase. In addition, both compounds showed a mixed type of inhibition against S. cerevisiae alpha-glucosidase and were very stable under thermal and acidic conditions up to 60 min. The K(I) and K(IS) values of compounds 1 and 2 were 0.44 and 0.22 mug/mL, and 0.39 and 0.13 mug/mL, respectively. Practical Application: One therapeutic approach for preventing diabetes mellitus is to retard the absorption of glucose via inhibition of alpha-glucosidase. In this study, 2 fatty acids with strong alpha-glucosidase-inhibitory activity, 7(Z)-octadecenoic acid and 7(Z),10(Z)-octadecadienoic acid, were purified and identified from sea cucumber. Therefore, sea cucumber fatty acids can potentially be developed as a novel natural nutraceutical for the management of type-2 diabetes.CI - (c) 2011 Institute of Food Technologists(R.
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