Take our Survey

Reference: Zhang Q, et al. (2012) Improved ethanol production of a newly isolated thermotolerant Saccharomyces cerevisiae strain after high-energy-pulse-electron beam. J Appl Microbiol 112(2):280-8

Reference Help

Abstract

OBJECTIVE: To isolate thermotolerant Saccharomyces cerevisiae with high-energy-pulse-electron (HEPE) beam, to optimize the mutation strain fermentation conditions for ethanol production and to conduct a preliminary investigation into the thermotolerant mechanisms. RESULTS: After HEPE beam radiation, the thermotolerant S. cerevisiae strain Y43 was obtained at 45?C. Moreover, the fermentation conditions of mutant Y43 were optimized by L3(3) orthogonal experiment. The optimal glucose content and initial pH for fermentation were 20% g l(-1) and 4?5, respectively; peptone content was the most neglected important factor. Under this condition, ethanol production of Y43 was 83?1 g l(-1) after fermentation for 48 h at 43?C, and ethanol yield was 0?42 g g(-1), which was about 81?5% of the theoretical yield. The results also showed that the trehalose content and the expression of the genes MSN2, SSA3 and TPS1 in Y43 were higher than those in the original strain (YE0) under the same stress conditions. CONCLUSIONS: A genetically stable mutant strain with high ethanol yield under heat stress was obtained using HEPE. This mutant may be a suitable candidate for the industrial-scale ethanol production. CONCLUSIONS: High-energy-pulse-electron radiation is a new efficient technology in breeding micro-organisms. The mutant obtained in this work has the advantages in industrial ethanol production under thermostress. ? 2012 The Authors. Journal of Applied Microbiology ? 2012 The Society for Applied Microbiology.

Reference Type
Journal Article
Authors
Zhang Q, Fu Y, Wang Y, Han J, Lv J, Wang S
Primary Lit For
Additional Lit For
Review For

Interaction Annotations

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations

Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference