Reference: Quevedo O, et al. (2011) No role of homologous recombination in dealing with ?-lapachone cytotoxicity in yeast. Chem Res Toxicol 24(12):2106-8

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Abstract

?-Lapachone (?-lap) is a promising antitumoral agent. DNA base oxidation and alkylation are among the expected damages by ?-lap. Herein, we have explored the role that the homologous recombination pathway (HR), a critical DNA repair process in Saccharomyces cerevisiae, has in the cytotoxic profile of ?-lap. We have further compared ?-lap to the closely related compound menadione and the well-known alkylating agent methyl methanesulfonate (MMS). Surprisingly, we found that ?-lap does not trigger HR, as seen for (i) the mutant sensitivity profiles, (ii) concentration-dependent arrest profiles, (iii) absence of nuclear DNA repair factories, and (iv) frequency of recombination between direct repeats.

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Journal Article
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Quevedo O, Garcia-Luis J, Lorenzo-Castrillejo I, Machin F
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