Saccharomyces cerevisiae acquires its resistance to carboxylate weak organic acids by inducing a plasma membrane ABC transporter, Pdr12. These acids activate a Zn(II)2Cys6 zinc-finger transcription factor, War1, which in turn induces the PDR12 gene. Mutation of the four potential sites of serine/threonine phosphorylation within the War1 activation domain revealed that Pdr12 induction was lost with mutations S923A or S930A, but not with the corresponding phosphomimetic mutations S923D or S930D. However, phosphorylation at these two sites has not been detected by mass spectrometry, so it still remains uncertain whether these are true sites of phosphorylation or merely serines whose side-chain hydroxyls are necessary for the proper structuring of the War1 activation domain. Mutation S923A prevented the sorbate-induced hyperphosphorylation of War1, while S930A caused War1 to be in a constitutively hyperphosphorylated state, irrespective of weak acid stress. Screening of non-essential protein kinase mutants of yeast failed to identify a kinase required for Pdr12 induction, or War1 hyperphosphorylation, in response to sorbate treatment. However, the mrk1∆ mutant was identified as having an elevated Pdr12 level in the absence of sorbate stress.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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