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Reference: Yamanishi M, et al. (2011) TPS1 terminator increases mRNA and protein yield in a Saccharomyces cerevisiae expression system. Biosci Biotechnol Biochem 75(11):2234-6

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Abstract

Both terminators and promoters regulate gene expression. In Saccharomyces cerevisiae, the TPS1 terminator (TPS1t), coupled to a gene encoding a fluorescent protein, produced more transgenic mRNA and protein than did similar constructs containing other terminators, such as CYC1t, TDH3t, and PGK1t. This suggests that TPS1t can be used as a general terminator in the development of metabolically engineered yeast in high-yield systems.

Reference Type
Journal Article
Authors
Yamanishi M, Katahira S, Matsuyama T
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