Reference: Mott C and Symington LS (2011) RAD51-independent inverted-repeat recombination by a strand-annealing mechanism. DNA Repair (Amst) 10(4):408-15

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Abstract


Recombination between inverted repeats is RAD52 dependent, but reduced only modestly in the rad51Delta mutant. RAD59 is required for RAD51-independent inverted-repeat recombination, but no clear mechanism for how recombination occurs in the absence of RAD51 has emerged. Because Rad59 is thought to function as an accessory factor for the single-strand annealing activity of Rad52 one possible mechanism for spontaneous recombination could be by strand annealing between repeats at a stalled replication fork. Here we demonstrate the importance of the Rad52 single-strand annealing activity for generating recombinants by showing suppression of the rad52Delta, rad51Delta rad52Delta and rad52Delta rad59Delta inverted-repeat recombination defects by the rfa1-D228Y mutation. In addition, formation of recombinants in the rad51Delta mutant was sensitive to the distance between the inverted repeats, consistent with a replication-based mechanism. Deletion of RAD5 or RAD18, which are required for error-free post-replication repair, reduced the recombination rate in the rad59Delta mutant, but not in wild type. These data are consistent with RAD51-independent recombinants arising by a faulty template switch mechanism that is distinct from nascent strand template switching.CI - Copyright (c) 2011 Elsevier B.V. All rights reserved.

Reference Type
Journal Article | Research Support, N.I.H., Extramural
Authors
Mott C, Symington LS
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