Reference: Scherthan H and Adelfalk C (2011) Live cell imaging of meiotic chromosome dynamics in yeast. Methods Mol Biol 745:537-48

Reference Help

Abstract


Recombination in first meiotic prophase is initiated by endogenous breaks in double-stranded DNA (DSBs) which occurs during a time when chromosomes are remodeled and proteinaceous cores (axes) are assembled along their length. DSBs are instrumental in homologue recognition and underlie the crossovers that form between parental chromosomes to ensure genome haploidization during the following two successive meiotic divisions. Advances in fluorescence microscopy and genetic engineering of GFP-tagged fusion proteins have made it possible to observe the behavior of entire chromosomes and specific subregions in live cells of the yeast Saccharomyces cerevisiae. In meiosis we observed that telomeres are dynamic and move about the entire nuclear periphery, only interrupted by their fleeting clustering at the spindle pole body (the centrosome equivalent), known as bouquet formation. This mobility translates to whole chromosomes and nuclei during the entire prophase I. Here we describe a simple setup for live cell microscopy that we used to observe chromosome movements during a time when DSBs are formed and transform into crossover and non-crossover products.

Reference Type
Journal Article
Authors
Scherthan H, Adelfalk C
Primary Lit For
Additional Lit For
Review For

Interaction Annotations


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations


Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference