Take our Survey

Reference: Diffley JF (2010) The many faces of redundancy in DNA replication control. Cold Spring Harb Symp Quant Biol 75:135-42

Reference Help

Abstract


The large genomes of eukaryotic cells are replicated from multiple replication origins during S phase of the cell cycle. These origins are not activated synchronously at the beginning of S phase but, instead, fire throughout S phase according to a predetermined, cell-type-specific program. Ensuring that each origin is efficiently activated once and only once during each S phase is crucial for maintaining the integrity of the genome. This is achieved by a two-step mechanism. The first step, licensing, involves the loading of the Mcm2-7 proteins into pre-replicative complexes (pre-RCs) at origins by ORC, Cdc6, and Cdt1. Pre-RCs can only assemble at origins during G(1) phase, when cyclin-dependent kinase (CDK) activity is low because CDKs inhibit each pre-RC component individually. CDKs trigger initiation by phosphorylating two essential proteins, Sld2 and Sld3. A second protein kinase, Cdc7, along with its regulatory subunit, Dbf4, is also required for initiation. In response to DNA damage, origin firing is inhibited by a third protein kinase, Rad53, which phosphorylates and inhibits Sld3 and Dbf4. In this chapter, I describe these regulatory mechanisms in detail and explore the role of redundancy in the regulation of DNA replication, focusing on the budding yeast, Saccharomyces cerevisiae.

Reference Type
Journal Article
Authors
Diffley JF
Primary Lit For
Additional Lit For
Review For

Interaction Annotations


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations


Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference