Reference: Kim Y, et al. (2011) Redox regulation of the tumor suppressor PTEN by glutaredoxin 5 and Ycp4. Biochem Biophys Res Commun 407(1):175-80

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Abstract


Human PTEN (phosphatase and tensin homolog deleted on chromosome 10; a phosphatidylinositol 3-phosphatase) expressed in Saccharomyces cerevisiae was oxidized in a time- and H(2)O(2)-concentration-dependent manner. Oxidized hPTEN was reduced by cellular reductants as in human cells. The reduction rate of oxidized hPTEN was monitored in S. cerevisiae mutants in which the genes involved in redox homeostasis had been disrupted. Reduction of hPTEN was delayed in each of S. cerevisiae grx5Delta and ycp4Delta mutants. Expression of Grx5 and Ycp4 in each of the mutants rescued the reduction rate of oxidized hPTEN. Furthermore, an in vitro assay revealed that the human Grx5/GSH system efficiently catalyzed the reduction of oxidized hPTEN. These results suggest that the reduction of oxidized hPTEN is regulated by Grx5 and Ycp4.CI - Copyright (c) 2011. Published by Elsevier Inc.

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Kim Y, Chay KO, Song YB, Kim TY, Kim I, Han SJ, Ahn Y, Cho SH, Hoe KL, Ahn BW, ... Show all
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