Mitochondria of the yeast Saccharomyces cerevisiae contain enzymes Crd1p and Psd1p, which synthesize cardiolipin (CL) and phosphatidylethanolamine, respectively. A previous study indicated that crd1Delta is synthetically lethal with psd1Delta. In this study, to identify novel genes involved in CL metabolism, we searched for genes that genetically interact with Psd1p, and found that deletion of FMP30 encoding a mitochondrial inner membrane protein results in a synthetic growth defect with psd1Delta. Although fmp30Delta cells grew normally and exhibited a slightly decreased CL level, fmp30Deltapsd1Delta cells exhibited a severe growth defect and an about twenty-fold reduction in the CL level, as compared to the wild-type control. We found also that deletion of FMP30 caused a defect in mitochondrial morphology. Furthermore, FMP30 genetically interacted with seven mitochondrial morphology genes. These results indicated that Fmp30p is involved in the maintenance of mitochondrial morphology and required for the accumulation of a normal level of CL in the absence of mitochondrial phosphatidylethanolamine synthesis.CI - (c) 2011 Blackwell Publishing Ltd.
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