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Reference: Verho R, et al. (2011) Cloning of two genes (LAT1,2) encoding specific L: -arabinose transporters of the L: -arabinose fermenting yeast Ambrosiozyma monospora. Appl Biochem Biotechnol 164(5):604-11

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Abstract


We identified and characterized two genes, LAT1 and LAT2, which encode specific L: -arabinose transporters. The genes were identified in the L: -arabinose fermenting yeast Ambrosiozyma monospora. The yeast Saccharomyces cerevisiae had only very low L: -arabinose transport activity; however, when LAT1 or LAT2 was expressed, L: -arabinose transport was facilitated. When the LAT1 or LAT2 were expressed in an S. cerevisiae mutant where the main hexose transporters were deleted, the L: -arabinose transporters could not restore growth on D: -glucose, D: -fructose, D: -mannose or D: -galactose. This indicates that these sugars are not transported and suggests that the transporters are specific for L: -arabinose.

Reference Type
Journal Article
Authors
Verho R, Penttila M, Richard P
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