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Reference: Costello JL, et al. (2011) The C-terminal Region of the Exosome-associated Protein Rrp47 Is Specifically Required for Box C/D Small Nucleolar RNA 3'-Maturation. J Biol Chem 286(6):4535-43

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Abstract


Cells lacking the exosome-associated protein Rrp47 show similar defects in stable RNA processing to those observed in the absence of the catalytic subunit Rrp6, but the precise mechanism(s) by which Rrp47 functions together with Rrp6 remains unclear. Deletion complementation analyses defined an N- terminal region of Rrp47, largely coincident with the bioinformatically defined Sas10/C1D domain, which was sufficient for protein function in vivo. In vitro protein interaction studies demonstrated that this domain of Rrp47 binds the PMC2NT domain of Rrp6. Expression of the N-terminal domain of Rrp47 in yeast complemented most RNA processing defects associated with the rrp47 mutant but failed to complement the defect observed in 3' end maturation of box C/D snoRNAs. Consistent with these results, protein capture assays revealed an interaction between the C-terminal region of Rrp47 and the snoRNP proteins Nop56 and Nop58. Filter binding assays demonstrated that deletion of the lysine-rich sequence at the C-terminus of Rrp47 blocked RNA binding in vitro. Furthermore, a protein mutated both at the C-terminus and within the N-terminal domain showed a synergistic defect in RNA binding without impacting on its ability to interact with Rrp6. These studies provide evidence for a role of Rrp47 in registering snoRNP particle assembly, functionally characterise the Sas10/C1D domain of Rrp47 and show that both the C-terminus of Rrp47 and the N-terminal domain contribute to its RNA binding activity.

Reference Type
Journal Article
Authors
Costello JL, Stead JA, Feigenbutz M, Jones RM, Mitchell P
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