Reference: Nedeva TS, et al. (2004) Cu/Zn superoxide dismutase in yeast mitochondria - a general phenomenon. FEMS Microbiol Lett 230(1):19-25

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Abstract


Fermentative and respiratory yeast strains of genera Saccharomyces, Kluyveromyces, Pichia, Candida and Hansenula have been investigated for mitochondrial localization of Cu/Zn superoxide dismutase (SOD). Pure mitochondrial fractions were obtained and the specific activities of Cu/Zn and Mn SODs were measured in comparison with those in the corresponding cell-free extracts. The Cu/Zn SOD: Mn SOD ratio in mitochondria and crude extracts was calculated and was considered a specific characteristic of all tested strains. Electrophoretical visualization of SOD patterns provided evidence for possible migration of cytosolic Cu/Zn SOD to mitochondria. The characteristic Cu/Zn SOD profile in mitochondria of all tested strains suggested its ubiquity within the fermentative and respiratory yeasts.

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Journal Article
Authors
Nedeva TS, Petrova VY, Zamfirova DR, Stephanova EV, Kujumdzieva AV
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