The transcriptional activation response relies on a repertoire of transcriptional activators, which decipher regulatory information through their specific binding to cognate sequences, and their capacity to selectively recruit the components that constitute a given transcriptional complex. We have addressed the possibility of achieving novel transcriptional responses by the building up a new transcriptional regulator constituted by the Hap2-3-5-Gln3 hybrid modulator, harbouring the HAP complex polypeptides which constitute the DNA binding domain (Hap2-3-5) and the Gln3 activation domain, which customarily act in an uncombined fashion. Results presented herein show that GDH1 and ASN1 transcriptional activation under a nitrogen repressive condition is achieved through the action of the novel Hap2-3-5-Gln3 transcriptional regulator. We propose that the combination of Hap-DNA-binding and Gln3 activation domain results in the constitution of a hybrid modulator that elicits novel transcriptional response not evoked when these modulators act in a non-combinatorial fashion.
|Evidence ID||Analyze ID||Interactor||Interactor Systematic Name||Interactor||Interactor Systematic Name||Type||Assay||Annotation||Action||Modification||Phenotype||Source||Reference||Note|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Gene Ontology Term||Gene Ontology Term ID||Qualifier||Aspect||Method||Evidence||Source||Assigned On||Annotation Extension||Reference|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Phenotype||Experiment Type||Experiment Type Category||Mutant Information||Strain Background||Chemical||Details||Reference|
|Evidence ID||Analyze ID||Regulator||Regulator Systematic Name||Target||Target Systematic Name||Experiment||Assay||Construct||Conditions||Strain Background||Reference|