Reference: Gaspar ML, et al. (2011) Coordination of Storage Lipid Synthesis and Membrane Biogenesis: EVIDENCE FOR CROSS-TALK BETWEEN TRIACYLGLYCEROL METABOLISM AND PHOSPHATIDYLINOSITOL SYNTHESIS. J Biol Chem 286(3):1696-708

Reference Help

Abstract


Despite the importance of triacylglycerols (TAG) and steryl esters (SE) in phospholipid synthesis in cells transitioning from stationary-phase into active growth, there is no direct evidence for their requirement in synthesis of phosphatidylinositol (PI) or other membrane phospholipids in logarithmically growing yeast cells. We report that the dga1lro1are1are2 strain, which lacks the ability to synthesize both TAG and SE, is not able to sustain normal growth in the absence of inositol (Ino(-) phenotype) at 37 degrees C especially when choline is present. Unlike many other strains exhibiting an Ino(-) phenotype, the dga1lro1are1are2 strain does not display a defect in INO1 expression. However, the mutant exhibits slow recovery of PI content compared to wild type cells upon reintroduction of inositol into logarithmically growing cultures. The tgl3tgl4tgl5 strain, which is able to synthesize TAG but unable to mobilize it, also exhibits attenuated PI formation under these conditions. However, unlike dga1lro1are1are2, the tgl3tgl4tgl5 strain does not display an Ino(-) phenotype indicating that failure to mobilize TAG is not fully responsible for the growth defect of the dga1lro1are1are2 strain in the absence of inositol. Moreover, synthesis of phospholipids especially PI, is dramatically reduced in the dga1lro1are1are2 strain even when it is grown continuously in the presence of inositol. The mutant also utilizes a greater proportion of newly synthesized PI than wild type for the synthesis of inositol containing sphingolipids, especially in the absence of inositol. Thus, we conclude that storage lipid synthesis actively influences membrane phospholipid metabolism in logarithmically growing cells.

Reference Type
Journal Article
Authors
Gaspar ML, Hofbauer HF, Kohlwein SD, Henry SA
Primary Lit For
Additional Lit For
Review For

Interaction Annotations


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations


Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference