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Reference: Davey RP, et al. (2010) TURNIP: tracking unresolved nucleotide polymorphisms in large hard-to-assemble regions of repetitive DNA sequence. Bioinformatics 26(22):2908-9

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Abstract

SUMMARY: TURNIP comprises a suite of Perl scripts and modules that facilitates the resolution of microheterogeneity within hard-to-assemble repetitive DNA sequences. TURNIP was originally developed for the Saccharomyces Genome Resequencing Project (SGRP) within which the ribosomal DNA (rDNA) of 36 strains of S. cerevisiae were analysed to investigate the occurrence of potential polymorphisms. Here, 'partially resolved SNPs', or pSNPs, as well as indels, were found to be far more prevalent than previously suspected. More generally, the TURNIP software ascertains degrees of variation between large tandem repeats within a single locus, offering insights into mechanisms of genome stability and gene conversion in any organism for which genome sequence data are available. AVAILABILITY: The TURNIP source code, results files and online help are available at http://www.ncyc.co.uk/software/turnip.html CONTACT: robert.davey@bbsrc.ac.uk.

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Journal Article
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Davey RP, James SA, Dicks J, Roberts IN
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