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Reference: Strudwick N, et al. (2010) Ime1 and Ime2 Are Required for Pseudohyphal Growth of Saccharomyces cerevisiae on Nonfermentable Carbon Sources. Mol Cell Biol 30(23):5514-30

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Abstract


Pseudohyphal growth and meiosis are two differentiation responses to nitrogen starvation of diploid Saccharomyces cerevisiae. Nitrogen starvation in the presence of fermentable carbon sources is thought to induce pseudohyphal growth, whereas nitrogen and sugar starvation induces meiosis. In contrast to the genetic background routinely used to study pseudohyphal growth, Sigma1278b, non-fermentable carbon sources stimulate pseudohyphal growth in an efficiently sporulating strain, SK1. Pseudohyphal SK1 cells can exit pseudohyphal growth to complete meiosis. Two stimulators of meiosis, Ime1 and Ime2, are required for pseudohyphal growth of SK1 cells in the presence of non-fermentable carbon sources. Epistasis analysis suggests that Ime1 and Ime2 act in the same order in pseudohyphal growth as in meiosis. The different behaviors of SK1 and Sigma1278b strains are, in part, attributable to differences in cyclic AMP (cAMP) signaling. In contrast to Sigma1278b cells, hyperactivation of cAMP signaling using constitutive active Ras2(G19V) inhibited pseudohyphal growth in SK1 cells. Our data identify the SK1 genetic background as an alternative genetic background to study pseudohyphal growth and suggest overlap between signaling pathways controlling pseudohyphal growth and meiosis. Based on these findings we propose to include exit from pseudohyphal growth and entry in meiosis into the life cycle of S. cerevisiae.

Reference Type
Journal Article
Authors
Strudwick N, Brown M, Parmar VM, Schroder M
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