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Reference: Raab AM, et al. (2010) Metabolic engineering of Saccharomyces cerevisiae for the biotechnological production of succinic acid. Metab Eng 12(6):518-25

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Abstract


The production of bio-based succinic acid is receiving great attention, and several predominantly prokaryotic organisms have been evaluated for this purpose. In this study we report on the suitability of the highly acid- and osmotolerant yeast Saccharomyces cerevisiae as a succinic acid production host. We implemented a metabolic engineering strategy for the oxidative production of succinic acid in yeast by deletion of the genes SDH1, SDH2, IDH1, and IDP1. The engineered strains harbour a TCA cycle that is completely interrupted after the intermediates isocitrate and succinate. The strains show no serious growth constraints on glucose. In glucose-grown shake flask cultures, the quadruple deletion strain Deltasdh1Deltasdh2Deltaidh1Deltaidp1 produces succinic acid at a titer of 3.62g L(-1) (factor 4.8 compared to wild-type) at a yield of 0.11mol (mol glucose)(-1). Succinic acid is not accumulated intracellularly. This makes the yeast Saccharomyces cerevisiae a suitable and promising candidate for the biotechnological production of succinic acid on an industrial scale.CI - Copyright (c) 2010. Published by Elsevier Inc.

Reference Type
Journal Article
Authors
Raab AM, Gebhardt G, Bolotina N, Weuster-Botz D, Lang C
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