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Reference: Martel CM, et al. (2010) Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (Sterol 14{alpha}-Demethylase) Doxycycline-Regulated Mutant and Screening of the Azole Sensitivity of Aspergillus fumigatus Isoenzymes CYP51A and CYP51B. Antimicrob Agents Chemother 54(11):4920-3

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Abstract


Aspergillus fumigatus sterol 14alpha-demethylase isoenzymes CYP51A and B were heterologously expressed in a Saccharomyces cerevisiae mutant (YUG37:erg11) wherein native ERG11/CYP51 expression is controlled using a doxycycline-regulatable promoter. When cultured in the presence of doxycycline, recombinant YUG37:pcyp51A and YUG37:pcyp51B yeast were able to synthesize ergosterol and grow; a control strain harboring reverse-orientated cyp51A could not. YUG37:pcyp51A and YUG37:pcyp51B constructs showed identical sensitivity to itraconazole, posaconazole, clotrimazole and voriconazole. Conversely, YUG37:pcyp51A withstood 16-fold higher concentrations of fluconazole than YUG37:pcyp51B (8 and 0.5 mug ml(-1), respectively).

Reference Type
Journal Article
Authors
Martel CM, Parker JE, Warrilow AG, Rolley NJ, Kelly SL, Kelly DE
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