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Reference: Durairaj G, et al. (2010) Regulation of chromatin assembly/disassembly by Rtt109p, a histone H3 Lys56-specific acetyltransferase, in vivo. J Biol Chem 285(40):30472-9

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Abstract


Rtt109p, a histone acetyltransferase, associates with active genes, and acetylates lysine 56 (K56) on histone H3 in Saccharomyces cerevisae. However, the functional role of Rtt109p or H3 K56 acetylation in chromatin assembly/disassembly (and hence gene expression) immediately switching transcription on or off has not been clearly elucidated in vivo. Here, we show that Rtt109p promotes the eviction of histone H3 from a fast inducible yeast gene, GAL1, following transcriptional initiation via histone H3 K56 acetylation. Conversely, the deposition of histone H3 to GAL1 is significantly decreased in the presence of Rtt109p following transcriptional termination. Intriguingly, we also find that the deposition of histone H2B on pre-existing non-acetylated histone H3 K56 at GAL1 in Deltartt109 is significantly increased independently of histone H3 deposition immediately following transcriptional termination subsequent to a short induction. Consistently, histone H2B is not efficiently evicted from GAL1 in the absence of Rtt109p immediately following transcriptional induction. Furthermore, we show that the stimulated eviction or reduced deposition of histones by Rtt109p promotes the association of RNA polymerase II with GAL1, and hence the synthesis of GAL1 mRNA. These results, taken together, support the fact that Rtt109p regulates the deposition/eviction of histone H2B in addition to its role in stimulating histone H3 eviction, thus providing insight on chromatin assembly/disassembly, and hence gene expression in vivo.

Reference Type
Journal Article
Authors
Durairaj G, Chaurasia P, Lahudkar S, Malik S, Shukla A, Bhaumik SR
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