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Reference: Krajewski WA and Vassiliev OL (2010) The Saccharomyces cerevisiae Swi/Snf Complex Can Catalyze Formation of Dimeric Nucleosome Structures in Vitro. Biochemistry 49(31):6531-40

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Abstract

The Swi/Snf chromatin-remodeling complexes, human BAF/PBAF and yeast RSC, can catalyze formation of stably altered dimeric forms of nucleosomes. However, the ability to create remodeled dimers has not yet been reported for the S. cerevisiae Swi/Snf complex. Despite its similarity with the other Swi/Snf proteins, yeast Swi/Snf features certain structural and functional differences. This raises a question of whether ySwi/Snf can in fact catalyze formation of dimeric nucleosomes. The dimer formation was proposed to have a specific impact on chromatin-regulatory effects. Thus, the answer to the above question may be helpful to clarify the ySwi/Snf functions in vivo and to generalize the notions of the regulatory principles of Swi/Snf-family proteins. Here we describe ySwi/Snf-catalyzed formation of nucleosome dimers using mono- and dinucleosome templates assembled from purified histones and DNA of the high-affinity (601) nucleosome positioning sequence. We evaluated effects of nucleosome template geometry on the formation of altered dimers and assayed formation of altered nucleosomes pairs on reconstituted dinucleosomes.

Reference Type
Journal Article
Authors
Krajewski WA, Vassiliev OL
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