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Reference: Cardillo SB, et al. (2010) Uga3 and Uga35/Dal81 Transcription Factors Regulate UGA4 Transcription in Response to {gamma}-Aminobutyric Acid and Leucine. Eukaryot Cell 9(8):1262-71

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Abstract

The S. cerevisiae UGA4 gene encodes a permease capable of importing gamma-aminobutyric acid (GABA) and delta-aminolevulinic acid (ALA) into the cell. GABA dependent induction of this permease requires at least two positive acting proteins, the specific Uga3 factor and the pleiotropic Uga35/Dal81 factor. UGA4 is subjected to a very complex regulation and its induction is affected by the presence of extracellular amino acids; this effect is mediated by the plasma membrane amino acid sensor SPS. Our results show that leucine affects UGA4 induction and that the SPS sensor and the downstream effectors Stp1 and Stp2 participate in this regulation. Moreover, we found that Uga3 and Uga35/Dal81 transcription factors bind to UGA4 promoter in a GABA dependent manner and that this binding is impaired by the presence of leucine. We also found that Leu3 transcription factor regulates negatively UGA4 transcription, although it seems to be through an indirect mechanism.

Reference Type
Journal Article
Authors
Cardillo SB, Bermudez Moretti M, Correa Garcia S
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