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Reference: Kyriss MN, et al. (2010) Novel Functional Residues in the Core Domain of Histone H2B Regulate Yeast Gene Expression and Silencing and Affect the Response to DNA Damage. Mol Cell Biol 30(14):3503-18

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Abstract


Previous studies have identified novel modifications in the core fold domain of histone H2B, but relatively little is known about the function of these putative histone modification sites. We have mutated core modifiable residues that are conserved in yeast histone H2B, and characterized the effects of these mutants on yeast silencing, gene expression, and DNA damage response. We identified three histone H2B core modifiable residues as functionally important. We find that mutating H2B K49 in yeast confers a UV-sensitivity phenotype, and we confirm that the homologous residue in human histone H2B is acetylated and methylated in human cells. Our results also indicate that mutating H2B K111 impairs the response to MMS-induced DNA lesions and disrupts telomeric silencing and Sir4 binding. In contrast, mutating H2B R102 enhances silencing at yeast telomeres and the HML silent mating loci, and increases Sir4 binding to these regions. The H2B R102A mutant also represses the expression of endogenous genes adjacent to yeast telomeres, which is likely due to the ectopic spreading of the Sir complex in this mutant strain. We propose a structural model by which the H2B R102 and K111 regulate the binding of Sir complex to the nucleosome.

Reference Type
Journal Article
Authors
Kyriss MN, Jin Y, Gallegos IJ, Sanford JA, Wyrick JJ
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