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Reference: Lee D, et al. (2010) PUF3 Acceleration of Deadenylation in Vivo Can Operate Independently of CCR4 Activity, Possibly Involving Effects on the PAB1-mRNP Structure. J Mol Biol 399(4):562-575

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Abstract


The evolutionarily conserved PUF proteins stimulate CCR4 mRNA deadenylation through binding to 3' UTR sequences of specific mRNA. We have investigated the mechanisms by which PUF3 in Saccharomyces cerevisiae accelerates deadenylation of the COX17 mRNA. PUF3 was shown to affect PAN2 deadenylation of the COX17 mRNA independent of the presence of CCR4, suggesting that PUF3 acts though a general mechanism to affect deadenylation. Similarly, eIF4E, the cap-binding translation initiation factor, known to control CCR4 deadenylation, was shown to affect PAN2 activity in vivo. PUF3 was found to be required for eIF4E effects on COX17 deadenylation. Both eIF4E and PUF3 effects on deadenylation were shown, in turn, to necessitate a functional poly(A) binding protein (PAB1) in which removal of the RRM1 domain of PAB1 blocked both their effects on deadenylation. While removal of the proline-rich region (P domain) of PAB1 substantially reduces CCR4 deadenylation at non-PUF3 controlled mRNA and correspondingly blocked eIF4E effects on deadenylation, PUF3 essentially bypassed this P domain requirement. These results indicate that the PAB1-mRNP structure is critical for PUF3 action. We also found that multiple components of the CCR4-NOT deadenylase complex but not PAN2 interacted with PUF3. PUF3 appears, therefore, both to act independently of CCR4 activity, possibly through effects on PAB1-mRNP structure, and to be capable of retaining the CCR4-NOT complex.CI - Copyright (c) 2010 Elsevier Ltd. All rights reserved.

Reference Type
Journal Article
Authors
Lee D, Ohn T, Chiang YC, Quigley G, Yao G, Liu Y, Denis CL
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