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Reference: Li WF, et al. (2010) Structural basis for the different activities of yeast Grx1 and Grx2. Biochim Biophys Acta 1804(7):1542-1547

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Abstract

Yeast glutaredoxins Grx1 and Grx2 catalyze the reduction of both inter- and intra-molecular disulfide bonds using glutathione (GSH) as the electron donor. Although sharing the same dithiolic CPYC active site and a sequence identity of 64%, they have been proved to play different roles during oxidative stress and to possess different glutathione-disulfide reductase activities. To address the structural basis of these differences, we solved the crystal structures of Grx2 in oxidized and reduced forms, at 2.10A and 1.50A, respectively. With the Grx1 structures we previously reported, comparative structural analyses revealed that Grx1 and Grx2 share a similar GSH binding site, except for a single residue substitution from Asp89 in Grx1 to Ser123 in Grx2. Site-directed mutagenesis in combination with activity assays further proved this single residue variation is critical for the different activities of yeast Grx1 and Grx2.CI - Copyright (c) 2010. Published by Elsevier B.V.

Reference Type
Journal Article
Authors
Li WF, Yu J, Ma XX, Teng YB, Luo M, Tang YJ, Zhou CZ
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