Take our Survey

Reference: Varela E, et al. (2010) Mitotic expression of spo13 alters m-phase progression and nucleolar localization of cdc14 in budding yeast. Genetics 185(3):841-54

Reference Help

Abstract


Spo13 is a key meiosis-specific regulator required for centromere cohesion and co-orientation, and for progression through two nuclear divisions. We previously reported that it causes a G2/M arrest, and may delay the transition from late anaphase to G1, when overexpressed in mitosis. Yet its mechanism of action has remained elusive. Here we show that Spo13, which is phosphorylated and stabilized at G2/M in a Cdk/Clb-dependent manner, acts at two stages during mitotic cell division. Its G2/M arrest is reversible and largely independent of the Mad2 spindle checkpoint. Genome-wide profiling suggests that its anaphase delay results from inhibition of Cdc14 function, since mRNAs whose induction requires Cdc14 activation are reduced. We show that the Spo13-induced delay in anaphase correlates with Cdc14 phosphatase retention in the nucleolus and with cyclin B accumulation, which both impede anaphase exit. At the onset of arrest, Spo13 is primarily associated with the nucleolus, where Cdc14 also accumulates. Significantly, overexpression of Separase (Esp1), which promotes G2/M and anaphase progression, suppresses Spo13 effects in mitosis, arguing that Spo13 acts upstream or parallel to Esp1. Combined with reduced Pds1 and cyclin B degradation, our findings are consistent with a role for Spo13 in regulating APC function, which controls both G2/M and anaphase. Similar effects of Spo13 during meiotic MI may prevent cell cycle exit and initiation of DNA replication prior to MII, thus ensuring two successive chromosome segregation events without an intervening S phase.

Reference Type
Journal Article
Authors
Varela E, Schlecht U, Moina A, Fackenthal JD, Washburn BK, Niederhauser-Widerkehr C, Tsai-Pflugfelder M, Primig M, Gasser SM, Esposito RE
Primary Lit For
Additional Lit For
Review For

Interaction Annotations


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations


Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference