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Reference: Gartmann M, et al. (2010) Mechanism of eIF6-mediated inhibition of ribosomal subunit joining. J Biol Chem 285(20):14848-51

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Abstract

During the process of translation initiation, the essential eukaryotic translation initiation factor 6 (eIF6) is known to act as a ribosomal anti-association factor. However, a molecular understanding of the anti-association activity of eIF6 is still missing. Here we present the cryo-electron microscopy (cryo-EM) reconstruction of a complex of the large ribosomal subunit with eukaryotic eIF6 from S. cerevisiae. The structure reveals that the eIF6 binding site involves mainly rpL23 (L14p in E. coli). Based on our structural data, we propose that the mechanism of eIF6's anti-association activity is based on steric hindrance of intersubunit bridge formation around the dynamic bridge B6.

Reference Type
Journal Article
Authors
Gartmann M, Blau M, Armache JP, Mielke T, Topf M, Beckmann R
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