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Reference: Jimeno-Gonzalez S, et al. (2010) The Yeast 5'-3' Exonuclease Rat1p Functions during Transcription Elongation by RNA Polymerase II. Mol Cell 37(4):580-587

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Abstract

Termination of RNA polymerase II (RNAPII) transcription of protein-coding genes occurs downstream of cleavage/polyadenylation sites. According to the "torpedo" model, the 5'-3' exonuclease Rat1p/Xrn2p attacks the newly formed 5' end of the cleaved pre-mRNA, causing the still transcribing RNAPII to terminate. Here we demonstrate a similar role of S. cerevisiae Rat1p within the gene body. We find that the transcription processivity defect imposed on RNAPII by the rpb1-N488D mutation is corrected upon Rat1p inactivation. Importantly, Rat1p-dependent transcription termination occurs upstream the polyadenylation site. Genetic and biochemical evidence demonstrate that mRNA capping is defective in rpb1-N488D cells, which leads to increased levels of Rat1p all along the gene locus. Consistently, Rat1p-dependent RNAPII termination is also observed in the capping-deficient ceg1-63 strain. Our data suggest that Rat1p serves to terminate RNAPII molecules engaged in the production of uncapped RNA, regardless of their position on the gene locus.CI - Copyright (c) 2010 Elsevier Inc. All rights reserved.

Reference Type
Journal Article
Authors
Jimeno-Gonzalez S, Haaning LL, Malagon F, Jensen TH
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