Take our Survey

Reference: Gao L, et al. (2010) The yeast formin Bnr1p has two localization regions that show spatially and temporally distinct association with septin structures. Mol Biol Cell 21(7):1253-62

Reference Help

Abstract


Monitoring Editor: Charles Boone Formins are conserved eukaryotic proteins that direct the nucleation and elongation of unbranched actin filaments. The yeast formins, Bni1p and Bnr1p, assemble actin cables from the bud cortex and bud neck, respectively, to guide overall cell polarity. Here we examine the regions of Bnr1p responsible for bud neck localization. We define two nonoverlapping regions, Bnr1p-L1 (1-466) and Bnr1p-L2 (466-733) that can each localize to the bud neck independently of endogenous Bnr1p. Bnr1p-L1 and Bnr1p-L2 localize with septins at the bud neck, but show slightly differently spatial and temporal localization, reflecting the localization (Bnr1p-L1) or cell cycle timing (Bnr1p-L2) of full length Bnr1p. Bnr1p is known to be very stably localized at the bud neck, and both Bnr1p-L1 and Bnr1p-L2 also show relatively stable localization there. Overexpression of Bnr1p-L1, but not Bnr1p-L2, disrupts septin organization at the bud neck. Thus Bnr1p has two separable regions that each contribute to its bud neck localization.

Reference Type
Journal Article
Authors
Gao L, Liu W, Bretscher A
Primary Lit For
Additional Lit For
Review For

Interaction Annotations


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations


Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference