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Reference: Manfrini N, et al. (2010) Processing of meiotic DNA double strand breaks requires cyclin-dependent kinase and multiple nucleases. J Biol Chem 285(15):11628-37

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Abstract


Meiotic recombination requires the formation of programmed Spo11-dependent DNA double-strand breaks (DSBs). In S. cerevisiae, the Sae2 protein and the MRX complex are necessary to remove the covalently attached Spo11 protein from the DNA ends, which are then resected by so far unknown nucleases. Here, we demonstrate that phosphorylation of Sae2 Ser267 by the cyclin-dependent kinase (Cdk1) is required to initiate meiotic DSB resection by allowing Spo11 removal from DSB ends. This finding suggests that Cdk1 activity is required for the processing of Spo11-induced DSBs, thus providing a mechanism for coordinating DSB resection with progression through meiotic prophase. Furthermore, the helicase Sgs1 and the nucleases Exo1 and Dna2 participate in lengthening the 5-3 resection tracts during meiosis by controlling a step subsequent to Spo11 removal.

Reference Type
Journal Article
Authors
Manfrini N, Guerini I, Citterio A, Lucchini G, Longhese MP
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