Take our Survey

Reference: Hou J, et al. (2010) Metabolic Impact of Increased NADH Availability in Saccharomyces cerevisiae. Appl Environ Microbiol 76(3):851-9

Reference Help

Abstract

Engineering the level of metabolic cofactors to manipulate metabolic flux is emerging to be an attractive strategy for bioprocess applications. We present the metabolic consequences of increasing NADH in the cytosol of the mitochondria of Saccharomyces cerevisiae. In a strain that was disabled to metabolize formate, we overexpressed the native NAD(+)-dependent formate dehydrogenase either in the cytosol or directed it into the mitochondria by fusing it with the mitochondrial signal sequence from the CYB2 gene. Upon exposure to formate, the mutant strains readily consumed formate and induced fermentative metabolism even under glucose de-repressed conditions. Cytosolic overexpression of formate dehydrogenase resulted in the production of glycerol while when this enzyme was directed into the mitochondria, we observed glycerol and ethanol production. Clearly, these results point towards different compartmental regulation of redox homeostasis. When pulsed with formate, S. cerevisiae growing at a steady-state on glucose immediately consumed formate. However, formate consumption ceased after 20 minutes. Our analysis revealed that metabolites at key branch points of metabolic pathways were affect the most by the genetic perturbations, while the intracellular concentration of sugar phosphates were specifically affected by time. In conclusion, the results have implications in the design of metabolic networks in yeast for industrial applications.

Reference Type
Journal Article
Authors
Hou J, Scalcinati G, Oldiges M, Vemuri GN
Primary Lit For
Additional Lit For
Review For

Interaction Annotations

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations

Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference