Reference: Olmo VN and Grote E (2010) Prm1 functions as a disulfide-linked complex in yeast mating. J Biol Chem 285(4):2274-83

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Abstract


Prm1 is a pheromone induced membrane glycoprotein that promotes plasma membrane fusion in yeast mating pairs. HA-Prm1 migrates at twice its expected molecular weight on non-reducing SDS-PAGE gels and co-precipitates with Prm1-TAP, indicating that Prm1 is a disulfide-linked homodimer. The N-terminus of a plasma membrane localized GFP-Prm1 endocytic mutant projects into the cytoplasm, where it is protected from low pH quenching in live cells and from external protease in spheroplasts. In a revised topological map, Prm1 has four transmembrane domains and two large extracellular loops. Mutation of all four cysteines in the extracellular loops blocked disulfide bond formation and destabilized the Prm1 homodimer without preventing Prm1 transport to contact sites in mating pairs. Cys120 in loop 1 and Cys545 in loop 2 form disulfide crosslinks in the Prm1 homodimer and are required for fusion activity. Cys120 lies between a hydrophobic segment formerly thought to be a transmembrane domain and an amphipathic helix. An interaction between either of these regions and the opposing membrane could promote fusion.

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Journal Article
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Olmo VN, Grote E
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