Reference: Jin K, et al. (2010) Carboxylate Transporter Gene JEN1 from the Entomopathogenic Fungus Beauveria bassiana Is Involved in Conidiation and Virulence. Appl Environ Microbiol 76(1):254-63

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Abstract

Beauveria bassiana is an important entomopathogenic fungus widely used as a biological agent to control insect pests. A gene (BbJEN1) homologous to JEN1 encoding a carboxylate transporter in Saccharomyces cerevisiae was identified from a B. bassiana T-DNA insertional mutant. The disruption of the gene decreased the amount of carboxylate contents in hyphae, while increased the conidial yield. Whereas, overexpression of this transporter resulted in a significant increase of carboxylates, and decreased the conidial yield. BbJEN1 was strongly induced by insect cuticles and highly expressed in the hyphae penetrating insect cuticles not in hyphal bodies, suggesting that this gene is involved in the early stage of pathogenesis of B. bassiana. Bioassay result indicated that the disruption of BbJEN1 significantly reduced the virulence of B. bassiana to aphids. Compared to the wild type, DeltaBbJEN1 alkalinized the insect cuticle to a lesser extent. The alkalinization of the cuticle is a physiological signal triggering the production of pathogenicity. Therefore, we identified a new factor influencing virulence, which is responsible for the alkalinization of the insect cuticle and the initiation of fungal pathogenesis in insects.

Reference Type
Journal Article
Authors
Jin K, Zhang Y, Fang W, Luo Z, Zhou Y, Pei Y
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