Autophagy is triggered when organisms sense radical environmental changes including nutritional starvation. During autophagy, cytoplasmic components including organelles are enclosed within autophagosomes and are degraded upon lysosome/vacuole fusion. In the present study, we show that processing of GFP-tagged Atg8 can serve as a marker for autophagy in Schizosaccharomyces pombe. Using this marker, 13 Atg homologs were also found to be required for autophagy in fission yeast. In budding yeast, autophagy-deficient mutants are known to be sterile, whereas in fission yeast we found that up to 30% of autophagy-defective cells with amino acid auxotrophy were able to recover sporulation when an excess of required amino acid compounds was supplied. Furthermore, we found that approximately 15% of the autophagy-defective cells were also able to sporulate when a prototroph strain was exposed to nitrogen starvation, which suggested that fission yeast may store sufficient intracellular nitrogen to allow partial sporulation under nitrogen-limiting conditions, although the majority of the nitrogen source is supplied by autophagy. Monitoring of the sporulation process revealed that the process was blocked nonspecifically at various stages in the atg1Delta and atg12Delta mutants, possibly due to a shortage of amino acids. Taking advantage of this partial sporulation ability of fission yeast, we tried to reveal the existence of a recycling system for nitrogen sources during starvation.
|Evidence ID||Analyze ID||Interactor||Interactor Systematic Name||Interactor||Interactor Systematic Name||Type||Assay||Annotation||Action||Modification||Phenotype||Source||Reference||Note|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Gene Ontology Term||Gene Ontology Term ID||Qualifier||Aspect||Method||Evidence||Source||Assigned On||Reference||Annotation Extension|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Phenotype||Experiment Type||Experiment Type Category||Mutant Information||Strain Background||Chemical||Details||Reference|
|Evidence ID||Analyze ID||Regulator||Regulator Systematic Name||Target||Target Systematic Name||Experiment||Conditions||Strain||Source||Reference|