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Reference: Milman N, et al. (2009) Meiotic DNA double-strand break repair requires two nucleases, MRN and Ctp1, to produce a single size class of Rec12 (Spo11)-oligonucleotide complexes. Mol Cell Biol 29(22):5998-6005

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Abstract

Programmed DNA double-strand breaks (DSBs) in meiosis are formed by Spo11 (Rec12 in fission yeast), a topoisomerase II-like protein, which becomes covalently attached to DNA 5' ends. For DSB repair through homologous recombination, the protein must be removed from these DNA ends. We show here that Rec12 is endonucleolytically removed from DSB ends attached to a short oligonucleotide (Rec12-oligo complex), as is Spo11 in budding yeast. Fission yeast, however, has only one size-class of Rec12-oligo complexes, whereas budding yeast has two size-classes, suggesting different endonucleolytic regulatory mechanisms. Rec12-oligo generation strictly requires Ctp1 (Sae2 nuclease homolog), the Rad32 (Mre11) nuclease domain, and Rad50 of the MRN complex. Surprisingly, Nbs1 is not strictly required, indicating separable roles for the MRN subunits. Based on these and other data, we propose that Rad32 nuclease has the catalytic site for Rec12-oligo generation and is activated by Ctp1, which plays an additional role in meiotic recombination.

Reference Type
Journal Article
Authors
Milman N, Higuchi E, Smith GR
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