Reference: Juneau K, et al. (2009) Alternative splicing of PTC7 in Saccharomyces cerevisiae determines protein localization. Genetics 183(1):185-94

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Abstract


It is well established that higher eukaryotes use alternative splicing to increase proteome complexity. In contrast, Saccharomyces cerevisiae, a single cell eukaryote, conducts predominantly regulated splicing through retention of non-functional introns. In this article we describe our discovery of a functional intron in the PTC7 (YHR076W) gene that can be alternatively spliced to create two mRNAs that code for distinct proteins. These two proteins localize to different cellular compartments and have distinct cellular roles. The protein translated from the spliced mRNA localizes to the mitochondria and its expression is carbon-source dependent. In comparison, the protein translated from the unspliced mRNA contains a transmembrane domain, localizes to the nuclear envelope and mediates the toxic effects of Latrunculin A exposure. In conclusion, we identified a definitive example of functional alternative splicing in S. cerevisiae that confers a measurable fitness benefit.

Reference Type
Journal Article
Authors
Juneau K, Nislow C, Davis RW
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