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Reference: Wang Y, et al. (2009) Cloning, overproduction, purification, crystallization and preliminary X-ray diffraction analysis of yeast glutaredoxin Grx5. Acta Crystallogr Sect F Struct Biol Cryst Commun 65(Pt 6):651-3

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Abstract

Grx5 from the yeast Saccharomyces cerevisiae is a monothiol glutaredoxin that is involved in iron-sulfur cluster biogenesis. Here, yeast Grx5 was cloned and overproduced in Escherichia coli. The purified protein was crystallized using the hanging-drop vapour-diffusion method. Diffraction data for Grx5 were collected to 1.67 A resolution. The crystal of Grx5 belonged to space group R3, with unit-cell parameters a = b = 85.12, c = 48.95 A, alpha = beta = 90.00, gamma = 120.00 degrees.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Wang Y, He YX, Yu J, Zhou CZ
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