Penicillium marneffei is an intracellular fungal pathogen. Being resistant to the reactive oxygen species (ROS) produced by phagocytes is a key step to the surviving of P. marneffei within the phagocytes, as well as its invasion to the host. SKN7, a transcription factor, contributes to the oxidative stress response in yeasts. We cloned the SKN7 ortholog in P. marneffei and compared its transcription on both mycelial and yeast phase, identified its function by complement the S. cerevisiae skn7 disruptant strain. The result showed the full sequence of SKN7 gene was about 2.5 kb, open reading frame extended to 1,845 bp and encoded a putative protein of 614 amino acids. Comparative analysis of the nucleotide sequence of genomic DNA and cDNA confirmed the presence of four introns and two highly conserved HSF-DNA-bind and REC domain. The deduced amino acid sequence was homologous to SKN7 from other fungi. Further, P. marneffei cDNA can partly complement S. cerevisiae skn7 disruptant strain, which was not viable in the presence of 2.5 mM H2O2. The expression level of SKN7 on the two phases, however, had no difference. These results indicated that P. marneffei SKN7 could response to oxidative stress and it was not a phase-specific gene.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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