Amphiphysins are proteins thought to be involved in synaptic vesicle endocytosis. Amphiphysins share a common BAR domain, which can "sense" and/or bend membranes, and this function is believed to be essential for endocytosis. S. cerevisiae cells lacking the amphiphysin ortholog, Rvs161, are inviable when starved for glucose. Altering sphingolipid levels in rvs161 cells remediates this defect, but how lipid changes suppress remains to be elucidated. Here we show sugar starvation-induced death of rvs161 cells extends to other fermentable sugar carbon sources, and loss of sphingolipid metabolism suppresses these defects. In all cases, rvs161 cells respond to the starvation signal, elicit the appropriate transcriptional response, and properly localize the requisite sugar transporter(s). However, Rvs161 is required for transporter endocytosis. rvs161 cells accumulate transporters at the plasma membrane under conditions normally resulting in their endocytosis and degradation. Transporter endocytosis requires the endocytosis (endo) domain of Rvs161. Altering sphingolipid metabolism, through deleting the very long chain fatty acid elongase, SUR4, re-initiates transporter endocytosis in rvs161 and rvs161 endo(-) cells. Sphingolipid-dependent re-initiation of endocytosis requires the ubiquitin-regulating factors Doa1, Doa4, and Rsp5. In the case of Doa1, the PFU ubiquitin-binding motif is dispensable. Moreover, the conserved AAA-ATPase, Cdc48, and its accessory proteins, Shp1 and Ufd1, are required. Finally, rvs161 cells accumulate monoubiquitin and this defect is remediated by loss of SUR4. These results show that defects in sphingolipid metabolism result in re-initiation of ubiquitin-dependent sugar transporter endocytosis and suggest this event is necessary for suppressing the nutrient starvation-induced death of rvs161 cells.
|Evidence ID||Analyze ID||Interactor||Interactor Systematic Name||Interactor||Interactor Systematic Name||Type||Assay||Annotation||Action||Modification||Phenotype||Source||Reference||Note|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Gene Ontology Term||Gene Ontology Term ID||Qualifier||Aspect||Method||Evidence||Source||Assigned On||Annotation Extension||Reference|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Phenotype||Experiment Type||Experiment Type Category||Mutant Information||Strain Background||Chemical||Details||Reference|
|Evidence ID||Analyze ID||Regulator||Regulator Systematic Name||Target||Target Systematic Name||Experiment||Assay||Construct||Conditions||Strain Background||Reference|