Reference: Ferrari SR, et al. (2009) The Mei5-Sae3 Protein Complex Mediates Dmc1 Activity in Saccharomyces cerevisiae. J Biol Chem 284(18):11766-70

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Abstract


During homologous recombination, a number of proteins cooperate to catalyze the loading of recombinases onto single-stranded DNA. Single-stranded DNA binding proteins stimulate recombination by coating single-stranded DNA and keeping it free of secondary structure; however, in order for recombinases to load on single-stranded-DNA-binding protein-coated DNA, the activity of a class of proteins known as recombination mediators is required. Mediator proteins coordinate the handoff of single-stranded DNA from single-stranded DNA binding protein to recombinase. Here we show that a complex of Mei5 and Sae3 from Saccharomyces cerevisiae preferentially binds single-stranded DNA and relieves the inhibition of the strand assimilation and DNA binding abilities of the meiotic recombinase Dmc1 imposed by the single-stranded DNA binding protein RPA. Additionally, we demonstrate the physical interaction of Mei5-Sae3 with RPA. Our results, together with previous in vivo studies, indicate that Mei5-Sae3 is a mediator of Dmc1 assembly during meiotic recombination in S. cerevisiae.

Reference Type
Journal Article
Authors
Ferrari SR, Grubb J, Bishop DK
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