Reference: Pinskaya M, et al. (2009) Nucleosome remodeling and transcriptional repression are distinct functions of Isw1 in Saccharomyces cerevisiae. Mol Cell Biol 29(9):2419-30

Reference Help

Abstract


The SANT domain is a nucleosome recognition module found in transcriptional regulatory proteins, including chromatin modifying enzymes. It shows high functional degeneracy between species, varying in sequence and copy number. Here we investigate functions in vivo associated with two SANT motifs, SANT and SLIDE, in the yeast S. cerevisiae Isw1 chromatin remodeling ATPase. We show that differences in the primary structure of the SANT and SLIDE domains in yeast and D. melanogaster reflect their different functions. In yeast, the SLIDE domain required for histone interactions while this is a function of the SANT domain in flies. In yeast, both motifs are required for optimal association with chromatin and to form the Isw1b complex (Isw1, Ioc2, Ioc4). Moreover, nucleosome remodeling at MET16 is defective in strains lacking the SANT or SLIDE domains. In contrast, the SANT domain is dispensable for the interaction between Isw1 and Ioc3 in the Isw1a complex. We show that, although defective in nucleosome remodeling, Isw1 lacking the SANT domain is able to repress transcription initiation at the MET16 promoter. Thus chromatin remodeling and transcriptional repression are distinct activities of Isw1.

Reference Type
Journal Article
Authors
Pinskaya M, Nair A, Clynes D, Morillon A, Mellor J
Primary Lit For
Additional Lit For
Review For

Interaction Annotations


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations


Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference