We have previously shown that Saccharomyces cereuisiae mitochondria contain a full set of mitochondrial DNA-coded isoaccepting tRNAs. We report here the presence of two lysine tRNA isoacceptors in purified yeast mitochondria, only one of which (tRNA2Lys) is of mitochondrial origin. The other mitochondria-associated lysine tRNA (tRNA1Lys) does not hybridize with mitochondrial DNA (mtDNA) and elutes with one of the cytoplasmic lysine tRNA isoacceptors in the RPC-5 column chromatographic system. This tRNA was purified by two-dimensional polyacrylamide gel electrophoresis of in vivo 32P-labeled total mitochondrial tRNA. Sequence analysis showed that it corresponds to cytoplasmic tRNALys (anticodon C-U-U), whose primary structure has already been determined by Smith and coworkers [Smith, C. J., Teh, H. s.,Ley, A., & D?Obrenan, P. (1973) J. Biol. Chem. 248,4475-4485]. tRNA1Lys is present in tRNA preparations extracted from RNase-treated mitochondria as well as from submitochondrial fractions, such as inner-membrane or mitosol preparations. It is absent in the mitochondrial outer-membrane preparation. These results show that the presence of tRNA1Lys in mitochondria is not a result of cytoplasmic contamination and suggest strongly that it is imported from the cytoplasm. This cytoplasmic tRNA seems not to be involved in the transfer of lysine into mtDNA-coded polypeptide chains since (1) it is not acylated by the mitochondrial aminoacyl-tRNA ligase preparation and (2) mtDNA-coded mitochondrial tRNA2Lys recognizes both lysine codons. It is postulated that the presence of tRNA1Lys in yeast mitochondria results from active transport involving a specific receptor (other than mitochondrial lysyl-tRNA ligase). This tRNA may have a regulatory role, and its function could give an important insight into the relationship between the mitochondrial and the nuclear-cytoplasmic systems.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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