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Reference: Kalebina TS, et al. (2008) Amyloid-like properties of Saccharomyces cerevisiae cell wall glucantransferase Bgl2p: Prediction and experimental evidences. Prion 2(2):91-6

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Abstract


Glucantransferase Bgl2p is a major conserved cell wall constituent described for a wide range of yeast species. In the baker's yeast Saccharomyces cerevisiae it is the only non-covalently bound cell wall protein that cannot be released from cell walls by sequential SDS and trypsin treatment. It contains seven amyloidogenic determinants. Circular dichroism analysis and fluorescence spectroscopy with thioflavin T indicate the presence of beta-sheet structures in Bgl2p isolates. Bgl2p forms fibrils, a process that is enforced in the presence of other cell wall components. Thus the data obtained is the first evidence for amyloid-like properties of yeast cell wall protein-glucantransferase Bgl2p.

Reference Type
Journal Article
Authors
Kalebina TS, Plotnikova TA, Gorkovskii AA, Selyakh IO, Galzitskaya OV, Bezsonov EE, Gellissen G, Kulaev IS
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