Saccharomyces cerevisiae is an efficient biosorbant, used in winemaking to reduce the concentration of undesirable molecules such as fatty acids. Volatile phenols such as 4-ethylphenol, which causes a horsy smell in wine, are particular targets of this type of curative process. This study demonstrates that the sorption capacity of 4-ethylphenol by yeasts is greatly influenced by strain nature, methods, and medium used for biomass production and drying after harvesting. S. cerevisiae mutant strains with deletion of genes encoding specific proteins involved in cell-wall structure and composition were studied, and a major role for mannoproteins in 4-ethylphenol sorption was identified. It was confirmed that 4-ethylphenol sorption occurs at the surface of the yeast wall and that not all mannoproteins are determinants of sorption: the sorption capacity of cells with deletion of the Gas1p-encoding gene was 75% lower than that of wild type. Physicochemical properties of yeast cell surface have been also studied.
|Evidence ID||Analyze ID||Interactor||Interactor Systematic Name||Interactor||Interactor Systematic Name||Type||Assay||Annotation||Action||Modification||Phenotype||Source||Reference||Note|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Gene Ontology Term||Gene Ontology Term ID||Qualifier||Aspect||Method||Evidence||Source||Assigned On||Annotation Extension||Reference|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Phenotype||Experiment Type||Experiment Type Category||Mutant Information||Strain Background||Chemical||Details||Reference|
|Evidence ID||Analyze ID||Regulator||Regulator Systematic Name||Target||Target Systematic Name||Experiment||Assay||Construct||Conditions||Strain Background||Reference|