Reference: Warner JR and Gorenstein C (1978) The ribosomal proteins of Saccharomyces cerevisiae. Methods Cell Biol 20:45-60

Reference Help

Abstract


The chapter studies the ribosomal proteins of Succharomyces cerevisiae because it is possible to obtain mutants which are likely to involve ribosomal proteins and focuses on the coordinated regulation of their synthesis. This chapter describes methods of preparing and analyzing the ribosomal proteins of S. cerevisiae and points out certain properties that are shared by the ribosomal proteins of all eukaryotes. Ribosomal proteins are analyzed directly on one-dimensional sodium dodecyl sulfate (SDS) gels without removing RNA or on two systems of two-dimensional polyacrylamide gel. The first is based on the Kaltschmidt???Wittman system and employs 6 M urea at pH 8.6 in the first dimension and at pH 4.5 in the second dimension. The second is a modified version of the Mets and Bogorad system and employs 8 M urea at pH 5.0 in the first dimension and sodium SDS in the second dimension. The two systems are described separately, and the results then compared. For both gel systems the importance of using proteins free of RNA cannot be overemphasized. RNA binds to ribosomal proteins even in urea solutions and causes poor resolution and decreased yields.

Reference Type
Journal Article | Research Support, U.S. Gov't, P.H.S.
Authors
Warner JR, Gorenstein C
Primary Lit For
Additional Lit For
Review For

Interaction Annotations


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations


Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference