Reference: Welz-Voegele C and Jinks-Robertson S (2008) Sequence divergence impedes crossover more than noncrossover events during mitotic gap repair in yeast. Genetics 179(3):1251-62

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Abstract

Homologous recombination between dispersed repeated sequences is important in shaping eukaryotic genome structure, and such ectopic interactions are affected by repeat size and sequence identity. A transformation-based, gap-repair assay was used to examine the effect of 2% sequence divergence on the efficiency of mitotic double-strand break repair templated by chromosomal sequences in yeast. Because the repaired plasmid could either remain autonomous or integrate into the genome, the effect of sequence divergence on the crossover-noncrossover (CO-NCO) outcome was also examined. Finally, proteins important for regulating the CO-NCO outcome and for enforcing identity requirements during recombination were examined by transforming appropriate mutant strains. Results demonstrate that the basic CO-NCO outcome is regulated by the Rad1-Rad10 endonuclease and the Sgs1 and Srs2 helicases; that sequence divergence impedes CO to a much greater extent than NCO events; that an intact mismatch repair system is required for the discriminating identical and nonidentical repair templates; and that the Sgs1 and Srs2 helicases play additional, anti-recombination roles when the interacting sequences are not identical.

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Journal Article
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Welz-Voegele C, Jinks-Robertson S
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