Reference: Ho HL, et al. (2008) Involvement of Saccharomyces cerevisiae Avo3p/Tsc11p in maintaining TOR complex 2 integrity and coupling to downstream signaling. Eukaryot Cell 7(8):1328-43

Reference Help

Abstract


Target of rapamycin (TOR) proteins are Ser/Thr kinases serving a central role in cell growth control. TORs function in two conserved multi-protein complexes, TORC1 and TORC2, while mechanisms underlying their actions and regulation are not fully elucidated. Saccharomyces TORC2, containing Tor2p, Avo1p, Avo2p, Avo3p/Tsc11p, Bit61p and Lst8p, regulates cell integrity and actin organization. Two classes of avo3(ts) mutants we previously identified both display cell integrity and actin defects, yet one is suppressed by AVO1 while the other by AVO2 or SLM1, defining two TORC2 downstream signaling mechanisms, one Avo1p-mediated and the other Avo2p/Slm1p-mediated. Employing these mutants we explored Avo3p functions in TORC2 structure and signaling. By checking binary protein interactions using co-immunoprecipitation we discovered differentially affected TORC2 composition and recruitment of downstream effectors Slm1p and Slm2p in different avo3(ts) mutants. These molecular defects can only be corrected by expressing AVO3 but not suppressors, highlighting the role of Avo3p as a structural and signaling scaffold for TORC2. Phenotypic modifications of avo3(ts) mutants by deleting individual Rho1p-GTPase activating proteins indicate that two TORC2 downstream signaling branches converge onto Rho1p activation. Results also suggest that Avo2p/Slm1p-mediated signaling but not Avo1p-mediated signaling links to Rho1p activation specifically through the Rho1p-guanine nucleotide exchange factor Tus1p.

Reference Type
Journal Article
Authors
Ho HL, Lee HY, Liao HC, Chen MY
Primary Lit For
Additional Lit For
Review For

Interaction Annotations


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations


Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference