Take our Survey

Reference: Feldbrugge M, et al. (2008) The posttranscriptional machinery of Ustilago maydis. Fungal Genet Biol 45 Suppl 1:S40-6

Reference Help

Abstract

Eukaryotic gene expression begins with transcription and maturation of mRNAs in the nucleus and ends with their translation and degradation in the cytoplasm. Here, we present an inventory of the posttranscriptional machinery of Ustilago maydis that is based on the recently sequenced genome and its comprehensive manual annotation. We used the detailed knowledge available for Saccharomyces cerevisiae and higher eukaryotes to predict posttranscriptional components in this plant pathogen. The comparison to S. cerevisiae revealed that most core components are shared. Both fungi belong to the small group of organisms lacking components of the RNAi machinery. However, a striking difference exists at the level of splicing. U. maydis harbors substantially more intron-containing genes and this correlates with the presence of numerous splice components with human orthologues that are absent or less conserved in S. cerevisiae. In particular, U. maydis contains three out of four core proteins of the exon junction complex, which marks spliced exons and is involved in cytoplasmic mRNA transport. In this context, it is also remarkable that the U. maydis genome displays components involved in microtubule- rather than actin-dependent mRNA transport. Thus, U. maydis might serve as an attractive model system to gain novel insights into posttranscriptional processes.

Reference Type
Journal Article
Authors
Feldbrugge M, Zarnack K, Vollmeister E, Baumann S, Koepke J, Konig J, Munsterkotter M, Mannhaupt G
Primary Lit For
Additional Lit For
Review For

Interaction Annotations

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations

Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference