Temperature-sensitive mutations in seven genes (cdc 2, 6, 7, 9, 13, 14, and 15) of Saccharomyces cerevisiae confer a defect in nuclear division. Following a shift from the permissive to the restrictive temperature, asynchronous populations of these mutant cells accumulate uniformly in nuclear division. Depending upon the particular gene defect, mutants appear to terminate development at an early stage of nuclear division (cdc 2), at a medial stage (cdc 6, 7, 9, 13), or at a late stage (cdc 14, 15). Experiments employing temperature shifts, time-lapse photomicroscopy, and synchronous cultures permitted a determination of the time in the cell cycle at which each of the thermolabile gene products completes its function at the permissive temperature (the execution point) for the ensuing nuclear division. These studies reveal a program of gene activity required for nuclear division that commences at the beginning of the cell cycle. Subsequent to the block in nuclear division the mutant strains do not undergo cell separation or bud initiation. Apparently, nuclear division is normally a prerequisite for the latter two processes.
|Evidence ID||Analyze ID||Interactor||Interactor Systematic Name||Interactor||Interactor Systematic Name||Type||Assay||Annotation||Action||Modification||Phenotype||Source||Reference||Note|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Gene Ontology Term||Gene Ontology Term ID||Qualifier||Aspect||Method||Evidence||Source||Assigned On||Reference||Annotation Extension|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Phenotype||Experiment Type||Experiment Type Category||Mutant Information||Strain Background||Chemical||Details||Reference|
|Evidence ID||Analyze ID||Regulator||Regulator Systematic Name||Target||Target Systematic Name||Experiment||Conditions||Strain||Source||Reference|