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Reference: Mesecke N, et al. (2008) A Novel Group of Glutaredoxins in the cis-Golgi Critical for Oxidative Stress Resistance. Mol Biol Cell 19(6):2673-80

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Abstract


Monitoring Editor: Jeffrey Brodsky Glutaredoxins represent a ubiquitous family of proteins which catalyze the reduction of disulfide bonds in their substrate proteins by use of reduced glutathione. In an attempt to identify the full complement of glutaredoxins in baker's yeast, we found three so far uncharacterized glutaredoxin-like proteins which we named Grx6, Grx7 and Grx8. Grx6 and Grx7 represent closely related monothiol glutaredoxins which are synthesized with N-terminal signal sequences. Both proteins are located in the cis-Golgi thereby representing the first glutaredoxins found in a compartment of the secretory pathway. In contrast to formerly described monothiol glutaredoxins, Grx6 and Grx7 showed a high glutaredoxin activity in vitro. Grx6 and Grx7 overlap in their activity and deletion mutants lacking both proteins show growth defects and a strongly increased sensitivity toward oxidizing agents like hydrogen peroxide or diamide. Our observations suggest that Grx6 and Grx7 do not play a general role in the oxidative folding of proteins in the early secretory pathway but rather counteract the oxidation of specific thiol groups in substrate proteins.

Reference Type
Journal Article
Authors
Mesecke N, Spang A, Deponte M, Herrmann JM
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